Introduction
Lyophilized (freeze-dried) peptides are stable by design, but storage and handling still determine research integrity. This guide outlines best practices for temperature, light, humidity, and reconstitution so your RUO materials remain reliable throughout experiments.
What “Lyophilized” Means
Lyophilization removes water under low temperature and vacuum, stabilizing peptide structure for transport and storage. While more robust than solutions, lyophilized peptides still degrade if exposed to heat, moisture, or repeated temperature cycling.
Storage Temperature Targets
- Long-term: −20 °C or lower; airtight and desiccated.
- Medium-term (weeks): 2–8 °C if −20 °C is unavailable.
- Short-term (days): Room temperature only if protected from light and humidity.
Tip: Avoid frost-free freezers for long-term storage—they cycle temperatures.
Light & Humidity Control
- Store vials in opaque secondary containers or foil pouches.
- Keep desiccant in the secondary container; replace if saturated.
- Minimize bench exposure; recap promptly after weighing or aliquoting.
Minimizing Freeze–Thaw Stress
Repeated freeze–thaw cycles accelerate degradation. Plan ahead:
- Remove only the vial(s) needed for that session.
- If frequent access is expected, pre-aliquot under low humidity, then refreeze.
Reconstitution Guidelines (RUO)
- Use sterile, appropriate solvent per protocol.
- Gently swirl—avoid aggressive vortexing.
- Record date, solvent, final concentration, and lot number.
- After reconstitution, aliquot and freeze to avoid repeat freeze–thaws (per SOP).
Labeling & Documentation
- Label: compound, lot, concentration (if reconstituted), date, initials.
- Keep an inventory that maps lot → storage location → experiment for reproducibility.
Shipping & Receiving Checklist
- Inspect outer box and inner packaging on delivery.
- Confirm intact tamper-evident seals and correct lot numbers.
- Photograph packaging and labels before filing—useful for audits and support.
RUO Reminder
All peptides supplied by Priority Aminos LLC are Research Use Only—not for human or veterinary use, diagnostic, or therapeutic applications.
Conclusion
With consistent cold storage, light/humidity control, and careful reconstitution, lyophilized peptides remain fit for purpose across studies.
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